9/13/2023 0 Comments Negative tb test resultsThis study was approved by the ethics committee of Ankang Central Hospital (ECACH-2016011). TB in active TB (ATB), with the goal of providing more reference evidence for the accurate application of T-SPOT. Our main objective based on population analysis is to identify factors associated with different antigen quantification results of T-SPOT. As a result, further research is urgently needed. In addition, there is a lack of quantitative examination of the association between spot-forming cells (SFCs) within PBMCs and clinical and laboratory characteristics. Some of these studies were qualitative studies with small samples and did not consider the possibility that different antigen risk factors might have confounded or biased the results. The association between IGRA and age, body mass index (BMI) and reduced lymphocyte levels is inconsistent among previous studies. Previous studies reported that negative bacteria in sputum, hypoproteinemia, combined HIV infection, anti-TB treatment, medical history, anemia, diabetes, parasitic infections, noncavitary lesions in the lung, fall and winter seasons, increased human leukocyte antigen DRB1–0701 allele and non-Hispanic white or Asian ethnicity are risk factors for false negative IGRA. IGRA still has a certain false negative rate among patients with tuberculosis (TB). TB) is the main IGRA test method it provides intuitive and reproducible results and quantitatively reflects the number of IFN-γ secreting cells in preparations of PBMCs. Currently, the T cell spot test for tuberculosis infection (T-SPOT. This test is not affected by Bacillus Calmette-Guerin (BCG) vaccination, a feature that is very beneficial in countries such as China in which general BCG vaccination is practiced. In principle, IGRA determines whether the subject is infected with MTB by examination of the levels of released γ-interferon (IFN-γ) after stimulation of whole blood or peripheral blood mononuclear cells (PBMCs) with MTB-specific antigen. As a new adjuvant method for the diagnosis of MTB infection, IGRA has been widely applied and accepted clinically. The interferon-gamma release assay (IGRA) represents one of the most important advances in the immunodiagnosis of Mycobacterium tuberculosis (MTB) infection in the past two decades. TB-assisted diagnosis of patients with active tuberculosis, previous treatment, decreased CD4+ and platelet count might lead to the decreased SFCs to ESAT-6, decreased alpha-2 globulin and high platelet distribution width might lead to the decreased SFCs to CFP-10, decreased albumin-globulin ratio, CD4+ and CD8+ might lead to an increase in the false negative rate of the T-SPOT. Albumin-globulin ratio, CD4+ and CD8+ were independent risk factors for false negative T-SPOT. The quantification graph showed that quantified SFCs to ESAT-6 or CFP-10 grading had a linear correlation with risk factors. Their importance levels were 0.287 and 0.247, respectively, with a total of 0.534. The SFCs to CFP-10 regression model also had statistical significance ( P < 0.001) platelet distribution width and alpha-2 globulin were its independent risk factors (all P < 0.05). Their importance levels were 0.095, 0.596 and 0.100, respectively, with a total of 0.791. The results showed that the SFCs to ESAT-6 regression model had statistical significance ( P < 0.001) and that previous treatment and CD4+ and platelet counts were its independent risk factors (all P < 0.05). TB) was analyzed by the exact logistic regression model. The combination of ESAT-6 and CFP-10 (i.e., T-SPOT. Using the SFCs to ESAT-6 or CFP-10 levels as dependent variables, variables with statistical significance in the univariate analysis were subjected to optimal scaling regression analysis. We retrospectively analyzed the data of 360 patients who met the inclusion criteria. The purpose of this study was to investigate the factors affecting quantified spot-forming cells (SFCs) to early secreted antigenic target 6 kDa (ESAT-6) or culture filtrate protein 10 kDa (CFP-10) in patients with active tuberculosis. However, more precise quantitative studies are lacking. TB) is associated with many risk factors in tuberculosis patients. Previous qualitative studies suggested that the false negative rate of the T cell spot test for tuberculosis infection (T-SPOT.
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