![]() (F) Total photon of MeLRR-overexpression cassava and the pEGAD control leaves. The flg22-triggered ROS burst were measured using luminol-based assay using a GloMax 96 Microplate Luminometer. (E) Dynamics of ROS accumulation in response to flg22 elicitation in MeLRR-overexpression cassava and the pEGAD control leaves. (D) Cassava leaves were observed using a Coomassie brilliant blue imaging system Fusion FX7-826 apparatus (Vilber Lourmat, France). The relative transcript level of MePR1 in the pEGAD control leaves was normalized to 1.0. (C) The pathogenesis-related gene ( MePR1) transcript level was quantitatively analyzed by qRT-PCR at 1 dpi. (B) The number of Xam populations in MeLRR-overexpression cassava and the pEGAD control leaves at 0 and 1 dpi, respectively. Then, the cassava leaves were syringe infiltrated with 4 × 10 8 cfu/mL of pathogenic bacteria Xam used for disease resistance assay. The relative transcript levels of MeLRRs in the pEGAD control leaves was normalized to 1.0. (A) At 3 days later, the relative transcript levels of MeLRRs in MeLRR-overexpression cassava and the pEGAD control leaves. Cassava leaves inject with recombinant pEGAD plasmids and empty vector of Agrobacterium GV3101, respectively. Transient overexpression of MeLRRs improved disease resistance against cassava bacterial blight. Asterisks (*) indicate significant differences at p < 0.05. Multiple comparisons of total photon were calculated by Student’s t-test. (F) Total photon of MeLRR-silenced cassava and the pTRV control leaves. ![]() The flg22-triggered ROS burst were measured using luminol-based assay by a GloMax 96 Microplate Luminometer. (E) Dynamic of ROS accumulation in response to flg22 elicitation in MeLRR-silenced cassava and the pTRV control leaves. The relative transcript level of MePR1 in the pTRV control leaves was normalized to 1.0. (B) The number of Xam populations in MeLRR-silenced cassava and the pTRV control leaves at 0 and 1 dpi, respectively. Then, the new leaves were syringe infiltrated with 4 × 10 8 cfu/mL of pathogenic bacteria Xam used for disease resistance assay. (A) At 14 dpi, the new leaves were used for relative transcript levels of MeLRRs in MeLRR-silenced leaves and the pTRV control leaves. The VIGS of MeLRRs reduced disease resistance against cassava bacterial blight. NBS-LRR ROS cassava cassava bacterial blight resistance genes salicylic acid.Ĭopyright © 2022 Zhang, Ye, Liu, Sun, Li, Wu, Zhou and Wan. Our findings shed light on the molecular mechanism underlying the regulation of cassava resistance against Xam inoculation. tomato, Alternaria brassicicola, and Botrytis cinerea. These pathogenic microorganisms include Pseudomonas syringae pv. Additionally, we revealed that MeLRRs positively regulated disease resistance in Arabidopsis. During cassava- Xam interaction, MeLRRs positively regulated endogenous SA and reactive oxygen species (ROS) accumulation and pathogenesis-related gene 1 ( PR1) transcripts. Four MeLRR genes positively regulate cassava disease general resistance against Xam via virus-induced gene silencing (VIGS) and transient overexpression. In this study, we isolated four MeLRR genes and assessed their expression under salicylic acid (SA) treatment and Xam inoculation. However, the in vivo roles of NBS-LRR remain unclear in cassava ( Manihot esculenta). Genes encoding nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domains are among the most important disease resistance genes in plants that are specifically involved in the response to diverse pathogens. manihotis ( Xam) seriously affects cassava yield. ![]() Cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv.
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